Georgiy A. Belogurov
Ph.D., Docent

M.S.- Moscow State University, School of Chemistry, Russia, 1997

Ph.D. - University of Turku, Dept. of Biochemistry and Food Chemistry, Finland, 2005

Postdoc - Ohio State Univesity, Dept. of Microbiology, USA, 2006-2009

Principal investigator - University of Turku, Dept. of Biochemistry, Finland, 2009-

Research interests

Transcription, nucleic acids enzymes, enzyme kinetics, protein chemistry, phylogenomics, membrane proteins


University of Turku, Dept. of Biochemistry, Vatselankatu 2, Turku FIN-20014, Finland. E-mail: gebelo(at) tel.: +358-2-333-6855


Turku Collegium for Science and Medicine
National Graduate School in Informational and Structural Biology
Systems Biology Research Program


Molecular Mechanisms of Transcription (RNAP group homepage)


Turtola M., Mäkinen J.J., Belogurov G.A. (2018) Active site closure stabilizes the backtracked state of RNA polymerase. Nucleic Acids Res. 46(20):10870-10887. [PubMed]

Supplementary Figure S6 in 3D (WebGL in browser):  Option 1   Option 2

Sanz-Murillo M., Xu J., Belogurov G.A., Calvo O., Gil-Carton D., Moreno-Morcillo M., Wang D., Fernández-Tornero C. (2018) Structural basis of RNA polymerase I stalling at UV light-induced DNA damage. Proc. Natl. Acad. Sci. USA 115(36):8972-8977. [PubMed]

Nedialkov Y., Svetlov D., Belogurov G.A., Artsimovitch I. (2018) Locking the non-template DNA to control transcription. Mol. Microbiol. 109(4):445-457. [PubMed]

Figure 4 Model 1 in 3D (WebGL in browser)
Figure 4 Model 2 in 3D (WebGL in browser)
Figure 4 Model 3 in 3D (WebGL in browser)
Figure 4 Model 4 in 3D (WebGL in browser)

Artsimovitch I., Belogurov G.A. (2018) Uneven Braking Spins RNA Polymerase into a Pause. Mol. Cell 69(5):723-725 [PubMed]

Animated Figure 1 (GIF)

Palmu K., Rosenqvist P., Thapa K., Ilina Y., Siitonen V., Baral B., Mäkinen J., Belogurov G., Virta P., Niemi J., Metsä-Ketelä M. (2017) Discovery of the Showdomycin Gene Cluster from Streptomyces showdoensis ATCC 15227 Yields Insight into the Biosynthetic Logic of C-Nucleoside Antibiotics. ACS Chem. Biol. 12(6):1472-1477 [PubMed]

NandyMazumdar M., Nedialkov Y., Svetlov D., Sevostyanova A., Belogurov G.A., Artsimovitch I. (2016) RNA polymerase gate loop guides the nontemplate DNA strand in transcription complexes. Proc. Natl. Acad. Sci. USA 113(52):14994-14999 [PubMed]

Figure 1A in 3D (WebGL in browser)
Figure 1B in 3D (WebGL in browser)

Turtola M., Belogurov G.A., (2016) NusG inhibits RNA polymerase backtracking by stabilizing the minimal transcription bubble. eLife 5. pii: e18096. [eLife] [PubMed]

Figure 8C in 3D (WebGL in browser)

Maddalena L.L., Niederholtmeyer H., Turtola M., Swank Z.N., Belogurov G.A., Maerkl S.J. (2016) GreA and GreB Enhance Expression of Escherichia coli RNA Polymerase Promoters in a Reconstituted Transcription-Translation System. ACS Synth. Biol. 5(9):929-35. [PubMed]

Esyunina D., Turtola M., Pupov D., Bass I., Klimašauskas S., Belogurov G., Kulbachinskiy A. (2016) Lineage-specific variations in the trigger loop modulate RNA proofreading by bacterial RNA polymerases. Nucleic Acids Res. 44(3):1298-308.[PubMed]

Belogurov G.A., Artsimovitch I. (2015) Regulation of Transcript Elongation. Annu. Rev. Microbiol. 69. doi: 10.1146/annurev-micro-091014-104047 [Annu. Rev. Microbiol.] [PubMed]

Artsimovitch I., Belogurov G.A. (2015) Creative Math of RNA Polymerase III Termination: Sense Plus Antisense Makes More Sense. Mol. Cell 58(6):974-6. doi: 10.1016/j.molcel.2015.06.003. [PubMed] [Mol. Cell]

Malinen A.M., Turtola M., Belogurov G.A. (2015) Monitoring translocation of multisubunit RNA polymerase along the DNA with fluorescent base analogues. Methods Mol. Biol. 1276:31-51. doi 10.1007/978-1-4939-2392-2_3. [PubMed] [Springer]

Malinen A.M., NandyMazumdar M., Turtola M., Malmi H., Grocholski T., Artsimovitch I., Belogurov G.A. (2014) CBR antimicrobials alter coupling between the bridge helix and the ß subunit in RNA polymerase. Nat. Commun. 5:3408 doi: 10.1038/ncomms4408 [PubMed] [NCOMMS]

Kallio P., Patrikainen P., Belogurov G.A., Mäntsälä P., Yang K., Niemi J., Metsä-Ketelä M. (2013) Tracing the evolution of angucyclinone monooxygenases: structural determinants for C-12b hydroxylation and substrate inhibition in PgaE. Biochemistry 52(26):4507-16. [PubMed]

Malinen A.M., Turtola M., Parthiban M., Vainonen L., Johnson M.S., Belogurov G.A. (2012) Active site opening and closure control translocation of multisubunit RNA polymerase. Nucleic Acids Res. 40(15): 7442-51. [PubMed] [NAR]

Sevostyanova A., Belogurov G.A., Mooney R.A., Landick R., Artsimovitch I. (2011) The ß subunit gate loop is required for RNA polymerase modification by RfaH and NusG. Mol. Cell 43(2): 253-262 [PubMed]

Luoto H.H., Belogurov G.A., Baykov A.A., Lahti R., Malinen A.M. (2011) Na+-translocating membrane pyrophosphatases are widespread in the microbial world and evolutionarily precede H+-translocating pyrophosphatases. J. Biol. Chem. 286(24): 21633-42 [PubMed]

Belogurov G.A., Sevostyanova A., Svetlov V., Artsimovitch I. (2010). Functional regions of the N-terminal domain of the antiterminator RfaH. Mol. Microbiol. 76 (2): 286-301 [PubMed]

Belogurov, G.A., Mooney, R.A. Svetlov, V., Landick, R., Artsimovitch, I. (2009) Functional specialization of transcription elongation factors. EMBO J. 28(2):112-22 [PubMed]

Belogurov, G.A., Vassylyeva, M.N. Sevostyanova, A., Xiang, A., Lira, R., Webber, S., Klyuyev, S., Nudler, E., Artsimovitch, I., Vassylyev, D.G. (2009) Transcription inactivation through local refolding of the RNA polymerase structure. Nature 457(7227): 332-335 [PubMed]

Svetlov V., Belogurov G.A., Shabrova E., Vassylyev D.G., Artsimovitch I. (2007) Allosteric control of the RNA polymerase by the elongation factor RfaH. Nucleic Acids Res. 35(17):5694-705 [PubMed]

Jämsen J., Tuominen H., Salminen A., Belogurov G.A., Magretova N.N., Baykov A.A., Lahti R. (2007) A CBS domain-containing pyrophosphatase of Moorella thermoacetica is regulated by adenine nucleotides. Biochem J. 408(3):327-33 [PubMed]

Belogurov G.A., Vassylyeva M.N., Svetlov V., Klyuyev S., Grishin N.V., Vassylyev D.G., Artsimovitch I. (2007) Structural basis for converting a general transcription factor into an operon-specific virulence regulator. Mol Cell. 26(1):117-29 [PubMed]

Malinen A. M., Belogurov G. A., Baykov A. A., Lahti R. (2007) Na+-pyrophosphatase: a novel primary sodium pump.
Biochemistry 46(30):8872-8 [PubMed]

Belogurov, G. A., Malinen, A. M., Turkina, M. V., Jalonen, U., Rytkönen, K., Baykov, A. A., Lahti, R. (2005) Membrane-bound pyrophosphatase of
Thermotoga maritima requires sodium for activity. Biochemistry 44(6): 2088-2096 [PubMed]

Malinen, A. M., Belogurov, G. A., Salminen, M., Baykov, A. A. and Lahti, R. (2004) Elucidating the role of conserved glutamates in H+-pyrophosphatase of
Rhodospirillum rubrum. J. Biol. Chem. 279: 26811-26816 [PubMed]

Belogurov, G. A. and Lahti, R. (2002) A Lysine substitute for K+: A460K mutation eliminates K+-dependence in H+-pyrophosphatase of
Carboxydothermus hydrogenoformans. J. Biol. Chem. 277: 49651-49654 [PubMed]

Belogurov, G. A., Turkina, M. V., Penttinen, A., Huopalahti, S., Baykov, A. A. and Lahti, R. (2002) H+-Pyrophosphatase of
Rhodospirillum rubrum: High-yield expression in Escherichia coli and identification of the Cys residues responsible for inactivation by mersalyl. J. Biol. Chem. 277: 22209-22214 [PubMed]

Belogurov G. A., Fabrichniy I. P., Pohjanjoki P., Kasho V. N., Lehtihuhta E., Turkina M. V., Cooperman B. S., Goldman A., Baykov A.A., Lahti R. (2000) Catalytically important ionizations along the reaction pathway of yeast pyrophosphatase.
Biochemistry 39(45):13931-13938 [PubMed]